碳酸酐酶Ⅶ(CA7)活性蛋白
Active Carbonic Anhydrase VII (CA7)
CA-VII; CAVII; Carbonate dehydratase VII
- 編號UAPD074Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Carbonic anhydrase catalyzes the reversible reaction of CO2? H2O = HCO3-? H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Carbonic Anhydrase?VII encoded by the CA7 gene is a cytosolic protein predominantly expressed in the salivary gland. CA7 may have additional tissue distributions and functions. For example, studies with CA inhibitors provide evidence that human CA7 is the CA isozyme responsible for the anticonvulsant/antiepileptic activity of sulfonamides and sulfamates. In fact, CA7 has been shown in a rat model to act as a developmental switch in GABAergic signaling in neurons. The activity of recombinant mouse CA7 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA7 (diluted by assay buffer) to 50 μL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPD074Mu01 | 碳酸酐酶Ⅶ(CA7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPD074Mu01 | 碳酸酐酶Ⅶ(CA7)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAD074Mu01 | 碳酸酐酶Ⅶ(CA7)多克隆抗體 | IHC |
| ULAD074Mu71 | 碳酸酐酶Ⅶ(CA7)多克隆抗體(生物素標記) | WB; IHC; ICC. |
