轉(zhuǎn)鐵蛋白(TF)活性蛋白
Active Transferrin (TF)
TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
- 編號(hào)UAPC036Ca61
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無報(bào)價(jià) 暫無報(bào)價(jià) 暫無報(bào)價(jià) 暫無報(bào)價(jià) 暫無報(bào)價(jià)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Transferrin (TF) is one of members of anion-binding superfamily of proteins. It is synthesized as a 698 amino acid (aa) precursor, which is mainly in the liver and is present in high concentrations in the bloodstream.TF plays a fundamental role in iron metabolism, protecting tissues from the potentially harmful effects of free iron, which can generate reactive oxygen species and cause oxidative damage. To test the effect of TF on cell proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant dog TF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant dog TF for 72h. The result was shown in Figure 1. It was obvious that TF significantly increased cell viability of HepG2 cells. The ED50 of recombinant dog TF is 0.292 μg/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC036Ca02 | 轉(zhuǎn)鐵蛋白(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC036Ca03 | 轉(zhuǎn)鐵蛋白(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPC036Ca61 | 轉(zhuǎn)鐵蛋白(TF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC036Ca61 | 轉(zhuǎn)鐵蛋白(TF)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC036Ca01 | 轉(zhuǎn)鐵蛋白(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC036Ca01 | 轉(zhuǎn)鐵蛋白(TF)多克隆抗體 | WB,IHC |
| UMAC036Ca21 | 轉(zhuǎn)鐵蛋白(TF)單克隆抗體 | WB; IHC; ICC; IP. |
| USEC036Ca | 轉(zhuǎn)鐵蛋白(TF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCC036Ca | 轉(zhuǎn)鐵蛋白(TF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMC036Ca | 轉(zhuǎn)鐵蛋白(TF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance [PubMed: PMC3754973] |
| Physiological research | The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743] |
| Journal of?Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia [Pubmed:29654921] |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
| The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria | Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria [] |
| Haematologica | New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208] |
