瘦素(LEP)單克隆抗體

Monoclonal Antibody to Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

編號(hào)UMAA084Ra22
物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))D1
Ig亞型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 -
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/mL
且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價(jià)格 ¥ 330 771 ¥ 771 ¥ 1101 ¥ 2753 ¥ 11010
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

Western Blot; Samples: Lane1: Rat Mammary gland lysate; Lane2: Porcine Mammary gland lysate;
Primary Ab: 0.2μg/ml Mouse Anti-Rat LEP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: T-47D cell lysate;
Primary Ab: 0.2μg/ml Mouse Anti-Rat LEP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: 3T3-L1 cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Rat LEP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)LEP的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別LEP。

用法

Western blotting: 0.1-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
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Bioscience Reports	Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats [Pubmed:29784871]
Neurochemical?Research	The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents [Pubmed:29397535]
PLoS One	Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens [Pubmed:29373588]
Biochemical and Biophysical Research Communications	Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae) [Pubmed:29626477]
Alcohol?and?Alcoholism	Leptin Expression and Gene Methylation Patterns in Alcohol-Dependent Patients with Ethyltoxic Cirrhosis—Normalization After Liver Transplantation and Implications?… [Pubmed:29912265]
Iranian?Red?Crescent?Medical?Journal?	The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer [ircmj-20-03-13305.pdf]
Pakistan Journal of Zoology	Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. [:]
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Progress in Nutrition	Effect of fermented food consumption on biochemical parameters and adipokines levels [Doi: 0.23751/pn.v20i4.7374]
THE INDIAN JOURNAL OF VETERINARY SCIENCES & BIOTECHNOLOGY	THE INDIAN JOURNAL OF VETERINARY SCIENCES & BIOTECHNOLOGY []
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Nutrition in the Prevention and Treatment of Abdominal Obesity	Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig []
Scientific Reports	Obesity Aggravates Acute Pancreatitis via Damaging Intestinal Mucosal Barrier and Changing Microbiota Composition in Rats [Pubmed: 30635594]
Atherosclerotic diseases	Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome [Pubmed: 30775602]
PLoS One	Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit [Pubmed: 30763367]
Scientific Journal of Kurdistan University of Medical Sciences	Effect of High Intensity Interval Training (HIIT) on visceral and subcutaneous levels of leptin and plasma insulin and glucose in male Wistar rats []
PLOS?ONE	The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic?… [Pubmed: 30870482]
International Journal of Pharmaceutical Research & Allied Sciences	Osteoprotegerin Levels among Saudi Cardiovascular Patients. []
Journal of Ethnopharmacology	Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet []
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Arch Physiol Biochem	Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696]
Food & Function	A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese?… []
Nutrients	High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?… []
Developmental Dynamics	Puberty is a critical window for the impact of diet on mammary gland development in the rabbit [Pubmed: 31348557]
Adv Gerontol	The Effect of Different Types of Bariatric Surgery on Metabolic and Hormonal Parameters in Rats with a Decompensated Form of Type II Diabetes Mellitus [Pubmed: 31228372]
Proceedings of the Royal Society B: Biological Sciences	Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens [Pubmed: 31506054]
Food & Function	Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice [Pubmed: 31670366]
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Annals of Nutrition and Metabolism	Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity [Pubmed: 31775140]
Journal of Ethnopharmacology	Upregulation of MC4R and PPAR-α expression mediates the anti-obesity activity of Moringa oleifera Lam. in high-fat diet-induced obesity in rats [Pubmed: 31911179]
Journal of Natural Products	Prenylated Phenolic Compounds from the Aerial Parts of Glycyrrhiza uralensis as PTP1B and α-Glucosidase Inhibitors [Pubmed: 32196343]
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Frontiers in Endocrinology	RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034]
Ann Nutr Metab	Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789]
ANDROLOGIA	The effect of metformin treatment on the basal and gonadotropin‐stimulated steroidogenesis in male rats with type 2 diabetes mellitus [Pubmed: 32951228]
GENERAL AND COMPARATIVE ENDOCRINOLOGY	Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and?… [Pubmed: 32827511]
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Indian Journal of Forensic Medicine & Toxicology	Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
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Nat Commun	GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
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Biomed Res Int	Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model [Pubmed:35509713]
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Scientific Reports	SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions. []

留言咨詢

編號(hào)	適用物種：Rattus norvegicus (Rat，大鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA084Ra01	瘦素(LEP)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA084Ra01	瘦素(LEP)多克隆抗體	WB; IHC
ULAA084Ra71	瘦素(LEP)多克隆抗體(生物素標(biāo)記)	IHC
UMAA084Ra22	瘦素(LEP)單克隆抗體	WB
UMAA084Ra21	瘦素(LEP)單克隆抗體	WB
UMEA084Ra	瘦素(LEP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
USEA084Ra	瘦素(LEP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCA084Ra	瘦素(LEP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMA084Ra	瘦素(LEP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA084Ra01	瘦素(LEP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".